Semen analysis is vital for evaluating male reproductive status, especially in artificial insemination and fertility management of farm animals. In this study, semen quality collected from twelve New Zealand rabbits (Oryctolagus cuniculus) was assessed using three treatments: Treatment one was (semen with 100% tris-egg yolk), which served as the control; treatment two was (semen with 50% tris-egg yolk extender + 50% apple juice), and treatment three was (semen with 50% tris-egg yolk extender + 50% orange juice). Key parameters measured included spermatozoa, progressive motility, Average Path Velocity (APV), Curvilinear Velocity (VCL), Straight-line Velocity (VSL), linearity, livability, straightness, and Amplitude of Lateral Head (ALH). Results showed significant differences (p<0.05) at a 1:1 dilution ratio. The orange juice extender yielded the highest values for motility (92.67%), progressive motility (78.67%), and livability (93.92%), which was better the control tris-egg yolk extender, which had lower values (66.00, 48.33 and 92.41% respectively). However, increasing the dilution ratio to 1:2 caused a decline in semen quality across all parameters. Motility and progressive motility dropped to 76.67% and 66.33% with the orange juice extender, and even lower with the tris-egg yolk extender (48.00 and 35.50%). In conclusion, incorporating apple and orange juices into semen extender can enhance spermatozoa motility and overall semen quality in rabbits. Nonetheless, optimizing the dilution ratio is crucial to avoid quality reduction. These findings offer valuable insights for improving male fertility, reproductive efficiency, and productivity in rabbit farming and animal production.
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