Genetic Diversity and Population Structure of Heterobranchus bidorsalis Inferred from Cytochrome b gene Sequences

Abstract

Abstract

Mitochondria DNA (mtDNA) diversity Heterobranchus bidorsalis from Rivers Niger and
Benue were studied. The mtDNA Cytochrome-b gene was inferred using 1118bp partial
sequence of 27 and 8 samples from Rivers Niger and Benue Respectively. Low Guanine (13%)
and higher cytosine (31%) composition were revealed. Ten haplotypes(MG334177 to
MG334186)were distinguished. River Niger samples had seven unique haplotypes while two
haplotypes were shared among the two populations. Low nucleotide (0.0008 – 0.001) and high
(0.67 – 0.68) haplotype diversity were recorded .F 's (-0.02966 to 0.000) was non-significant (p ST
>0.05) among the populations. AMOVA(– 2.97%)shows no genetic structure between the
populations. Tajima's D and Fu's FS neutrality tests suggested recent population growth for the
populations. Phylogenetic reconstruction revealed monophyletic relationship among the
populations. Analysis of data demonstrated suitability of partial Cytochrome b sequence in
determining the genetic diversity in H. bidorsalis and provides new information for the species'
conservation and exploitation