ISOLATION AND IDENTIFICATION OF MULTI-DRUG-RESISTANT Pseudomonas ISOLATES FROM SHELLFISHES FROM A FISH MARKET IN LAGOS, NIGERIA
Abstract
Shellfishes are a delicacy worldwide and they are usually found at the bottom of water bodies. These aquatic resources can harbour pathogenic organisms, which cause food-borne infections when the shellfish is not adequately processed. The microbial quality of shellfish is therefore a major public health concern for marketers, processors and consumers. This study investigated the microbial quality of shrimp and crab samples from a fish market located in Lagos, Nigeria. Bacteria were isolated from shellfishes and characterized using standard microbial analysis techniques. The susceptibility of selected isolates to 15 antibiotics was determined using the modified disc diffusion method. Total heterotrophic count ranged from 1.20 × 107 to >1.0 × 108 cfu-1g, while the coliform count was between 4.08 × 104 and >1.0 x 108 cfu-1g. The isolates identified belonged to the seven genera: Pseudomonas, Proteus, Alcaligenes, Vibrio, Salmonella, Staphylococcus and Bacillus. Antibiotic susceptibility test showed that the three Pseudomonas spp. isolated were resistant to 3-6 different antibiotics, with multiple antibiotic resistance values ranging from 0.2 to 0.4. The presence of these organisms poses a potential health risk, as they can cause spoilage of shellfish, result in food-borne infections and confer antibiotic resistance to normal gut floral. There is therefore a need for regular monitoring of the safety of shellfishes sold in the market and creation of awareness on the dangers of improper handling and inadequate processing of shellfishes.
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